August 14, 2017 at 9:04 pm
#49233
Alchemist
Member
Below are the ingredients and the procedure
Suggested ingredients:
- Phenol crystals……………………………..1.2 g
- Ethanol 95% ………………………………..1.00 mL
- Sterile water for injection USP ………29.00 mL
Supplies:
- Empty sterile vials: 30 mL
- Syringes: 30 mL, 5 mL, 3 mL
- Sterile 0.22 micron filter (stable in phenol, eg, Acrodisc syringe filter with PTFE membrane)
- Needles: 18 ga, 20 ga
- Glass beaker
Suggested procedure:
- Weigh 1.2 gm phenol, place into the beaker.
- Add 1.0 mL Ethanol 95% to (1) and allow phenol to dissolve.
- Add 29 mL sterile water for injection (SWFI) to (2) with stirring.
- Pass mL Ethanol 95% through the filter to wet it, then flush with 5 mL SWFI.
- Draw phenol mixture in (3) into 30 mL syringe, add filter from (4),and add 20 ga needle.
- Prepare 30 mL empty sterile vial: wipe stopper with alcohol; insert 20 ga needle as air vent.
- Inject phenol solution into vial in (6). Remove needles and seal as desired.
- Label.
- Perform the bubble point filter integrity test
Precautions: Phenol is corrosive; steps 1 to 3 could be performed in a fume hood and the process moved into the cleanroom at step 4.
Reference:
